Time-Resolved Infrared Spectroscopy, Methods

Time-Resolved IR Spectroscopy and Methods

We have long-term experience with the development and application of various methodological approaches including time-resolved (step-scan, rapid-scan) FTIR spectroscopy, SEIRA (surface enhanced infrared absorption), (polarized) ATR (attenuated total reflection), fast (millisecond) mixing (stopped-flow). Non-equilibrium dynamics are studied with fast (nanosecond) laser-excited perturbation techniques (T-jump, pH-jump) and single wavelength detection using tunable quantum cascade lasers (QCL). Laser-pulses from the UV to the NIR are used to study light-induced reactions. Recently we extended our approaches to dual-comb QCL spectroscopy.

Selected Publications

Stritt, P., Jawurek, M., Hauser, K. "Mid-IR quantum cascade laser spectroscopy to resolve lipid dynamics during the photocycle of bacteriorhodopsin", J. Chem. Phys. 2023, 158, 154202, [pdf]

Fallah, M.A., Hauser, K. "Immobilization approaches can affect protein dynamics: a surface-enhanced infrared spectroscopic study on lipid-protein interactions", Biomaterials Science, 2019, 7, 3204-3212 [pdf]

Krüger, A., Bürkle, A., Mangerich, A., Hauser, K. "A combined approach of surface passivation and specific immobilization to study biomolecules by ATR-FTIR spectroscopy", Biomed. Spectros. and Imaging, 2018, 7, 25-33 [pdf]

Popp, A.^#, Scheerer, D.^#, Heck, B., Hauser, K. "Biomolecular dynamics studied with IR-spectroscopy using quantum cascade lasers combined with nanosecond perturbation techniques" Spectrochim Acta A Mol Biomol Spectrosc, 2017, 181, 192–199 PDF

Donten, M.L., Hassan, S., Popp, A., Halter, J., Hauser, K., Hamm, P. "pH-jump induced leucine zipper folding beyond the diffusion limit" J. Phys. Chem. B, 2015, 119, 1425–1432 PDF