Publications

Publications in 2024

• Targeted preparation and NMR spectroscopic characterization of Lys11-linked ubiquitin trimers.
  Immler F, Schneider T, Kovermann M.
  Chembiochem. 2024 Feb 1;25(3):e202300670.
   
• Including the Ensemble of Unstructured Conformations in the Analysis of Protein's Native State by High-Pressure NMR Spectroscopy.
  Berner F, Kovermann M.
  Angew. Chem. Int. Ed., accepted.

Publications in 2023

• The Mycobacterium tuberculosis protein tyrosine phosphatase MptpA features a pH dependent activity overlapping the bacterium sensitivity to acidic conditions.
  Kovermann M, Stefan A, Palazzetti C, Immler F, Piaz FD, Bernardi L, Cimone V, Bellone ML, Hochkoeppler A.
  Biochimie 2023, 213: 66-81.
   
• Multi-dimensional Methods in Biological NMR.
  Schneider T, Kovermann M.
  Two‐Dimensional (2D) NMR Methods 2023, 333-364, John Wiley & Sons, Inc. (book chapter)
   
• Distinct growth regimes of a-synuclein amyloid elongation.
  Horvath I, Welte H, Schmit J, Kovermann M, Wittung-Stafshede P.
  Biophys. J. 2023, 122: 1-8.
   
• Specifying conformational heterogeneity of multi-domain proteins at atomic resolution.
  Schneider T, Sawade K, Berner F, Peter C, Kovermann M.
  Structure 2023, 31: 1259-1274.
  Deciphering the molecular dynamics of complex proteins.

Publications in 2022

• Lipid-mediated activation of plasma membrane-localized deubiquitylating enzymes regulate endosomal degradation.
  Vogel K, Bläske T, Nagel MK, Globisch C, Maguire S, Mattes L, Gude C, Kovermann M, Hauser K, Peter C, Isono E.
  Nat. Commun. 2022, 13:6897.
   
• Electrostatic and steric effects underlie acetylation-induced changes in ubiquitin structure and function.
  Kienle SM, Schneider T, Stuber K, Globisch C, Jansen J, Stengel F, Peter C, Marx A, Kovermann M, Scheffner M.
  Nat. Commun. 2022, 13:5435.
   
• Identification of novel functional mini-receptors by combinatorial screening of split-WW domains.
  Neitz H, Paul NB, Lindner C, Graebner R, Häge F, Kovermann M, Thomas F.
  Chem. Sci. 2022, 13:9079-9090.
   
• A switchable zinc(II)-responsive globular β-sheet peptide.
  Pham TL, Kovermann M, Thomas F.
  ACS Synth. Biol. 2022, 11:254-264.
   
• Completing the family of human Eps15 homology domains: Solution structure of the internal Eps15 homology domain of γ-synergin.
  Kovermann M, Weininger U, Löw C.
  Prot. Sci. 2022, 31:811-821.

Publications in 2021

• Structural and functional consequences of NEDD8 phosphorylation.
  Stuber K, Schneider T, Werner J, Kovermann M, Marx A, Scheffner M.
  Nat. Commun. 2021, 12: 5939.
   
• Impact of crowded environments on binding between protein and single-stranded DNA.
  Köhn B, Schwarz P, Wittung-Stafshede P, Kovermann M.
  Sci. Rep. 2021, 11:17682.
   
• Fluorine NMR spectroscopy enables to quantify the affinity between DNA and proteins in cell lysate.
  Welte H, Sinn P, Kovermann M.
  ChemBioChem 2021, 22:2973-2980.
   
• Molecular characterisation of titin N2A and its binding of CARP reveals a titin/actin cross-linking mechanism.
  Zhou T, Fleming JR, Lange S, Hessel A, Bogomolova J, Stronczek C, Grundei D, Ghassemian M, Biju A, Börgeson E, Bullard B, Linke W, Che J, Kovermann M, Mayans O.
  J. Mol. Biol. 2021, 433:166901.
   
• Template-assisted design of monomeric polyQ models to unravel the unique role of glutamine side chains in disease-related aggregation.
  Siu H-W, Heck B, Kovermann M, Hauser K.
  Chem. Sci. 2021, 12:412-426.

Publications in 2020

• All atom insights into the impact of crowded environments on protein stability by NMR spectroscopy.
  Köhn B, Kovermann M.
  Nat. Commun. 2020, 11:5760.
   
• Synthesis of nickel hexacyanoferrate nanocubes with tuneable dimensions via temperature-controlled Ni2+-citrate complexation.
  Keßler S, González-Rubio G, Reinalter E, Kovermann M, Cölfen H.
  Chem. Commun. 2020, 56:14439-14442.
   
• Insights into Protein Stability in Cell Lysate by 19F NMR Spectroscopy.
  Welte H, Kovermann M.
  ChemBioChem 2020, 21:3575-3579.
   
• What does fluorine do to a protein? Thermodynamic, and highly-resolved structural insights into fluorine-labelled variants of the cold shock protein.
  Welte H, Zhou T, Mihajlenko X, Mayans O, Kovermann M.
  Sci. Rep. 2020, 10:2640.

Publications in 2019

• Conformational and functional characterization of artificially conjugated non-canonical ubiquitin dimers.
  Schneider T, Berg A, Ulusoy Z, Gamerdinger M, Peter C, Kovermann M.
  Sci. Rep. 2019, 9:19991.
   
• Liquid Metastable Precursors of Ibuprofen as Aqueous Nucleation Intermediates.
  Wiedenbeck E, Kovermann M, Gebauer D, Cölfen H.
  Angew. Chem. Int. Ed. 2019, 58:19103-19109.
   
• Structural and catalytic insights into HoLaMa, a derivative of Klenow DNA polymerase lacking the proofreading domain.
  Kovermann M, Stefan A, Castaldo A, Caramia S, Hochkoeppler A.
  PlosOne 2019, 14(4):e0215411.
   
• Crowding-induced elongated conformation of urea-unfolded apoazurin: investigating the role of crowder shape in silico.
  Zegarra F, Homouz D, Gasic A, Babel L, Kovermann M, Wittung-Stafshede P, Cheung, M.
  J. Phys. Chem. B 2019, 123:3607-3617.
   
• Structural and dynamic characterization of artificially linked ubiquitin dimers by NMR spectroscopy.
  Zhao X, Mißun M, Schneider T, Müller F, Lutz J, Scheffner M, Marx A, Kovermann M.
  ChemBioChem 2019, 20:1772-1777.
   
• Targeted expression and purification of fluorine labelled cold shock protein B by using an auxotrophic strategy.
  Welte H, Kovermann M.
  Prot. Expr. Purif. 2019, 157:86-91.
   
• Macromolecular crowding tunes protein stability by manipulating solvent accessibility.
  Köhn B, Kovermann M.
  ChemBioChem 2019, 20(6):759-763.

Publications in 2018

• A Luminal Loop of Wilson Disease Protein Binds Copper and Is Required for Protein Activity.
  Köhn B, Ponnandai Shanmugavel K, Wu M, Kovermann M, Wittung-Stafshede P.
  Biophys. J. 2018, 115:1-12.

Publications in 2017

• Structure-Based Insights into the Dynamics and Function of Two Domain SlpA from Escherichia coli.
  Geitner AJ, Weininger U,  Paulsen H, Balbach J, Kovermann M.
  Biochemistry 2017, 56:6533-6543.
   
• Structural basis for ligand binding to an enzyme by a conformational selection pathway.
  Kovermann M, Grundström C, Grundström C, Elisabeth Sauer-Eriksson A, Sauer UH, Wolf-Watz M.
  Proc. Natl. Acad. Sci. 2017, 114:6298-6303.

Publications in 2016

• Linkage between Fitness of Yeast Cells and Adenylate Kinase Catalysis.
  Tukenmez H, Magnussen H, Kovermann M, Byström A, Wolf-Watz M.
  PLoS One 2016, 11:e0163115.
   
• Protein dynamics and function from solution state NMR spectroscopy.
  Kovermann M, Rogne P, Wolf-Watz M.
  Q. Rev. Biophys. 2016, e6:1-43.
   
• The C-terminus of human copper importer, Ctr1, acts as a binding site and transfers copper to Atox1.
  Kahra D, Kovermann M, Wittung-Stafshede, P.
  Biophys. J. 2016, 110:95-102.

Publications in 2015

• High-resolution structures of the d-alanyl carrier protein (Dcp) DltC from Bacillus subtilis reveal equivalent conformations of apo- and holo-forms.
  Zimmermann S, Pfennig S, Neumann P, Yonus H, Weininger U, Kovermann M, Balbach J, Stubbs MT.
  FEBS Lett. 2015, 589:2283-2289.
   
• Structural basis for catalytically restrictive dynamics of a high-energy enzyme state.
  Kovermann M, Ådén J, Grundström C, Elisabeth Sauer-Eriksson A, Sauer UH, Wolf-Watz M.
  Nat. Commun. 2015, 6:7644.
   
• Novel sulfated phosphoglycolipids from Natronomonas moolapensis.
  Hoffmann A, Kovermann M, Oberwinkler T, Siedler F, Cortina NS, Balbach J, Oesterhelt D.
  Chem. Phys. Lipids 2015, 191:8-15.
   
• Solution structure of the PsIAA4 oligomerization domain reveals interaction modes for transcription factors in early auxin response.
  Dinesh DC, Kovermann M, Gopalswamy M, Hellmuth A, Calderón Villalobos LI, Lilie H, Balbach J, Abel S.
  Proc. Natl. Acad. Sci. 2015, 112:6230-6235.
   
• Human cytoplasmic copper chaperones Atox1 and CCS exchange copper ions in vitro.
  Petzoldt S, Kahra D, Kovermann M, Dingeldein AP, Niemiec MS, Ådén J, Wittung-Stafshede P.
  Biometals 2015, 28:577-85.

Publications in 2014

• Initiation of RNA synthesis by the hepatitis C virus RNA-dependent RNA polymerase is affected by the structure of the RNA template.
  Reich S, Kovermann M, Lilie H, Knick P, Geissler R, Golbik RP, Balbach J, Behrens SE.
  Biochemistry 2014, 53:7002-7012.
   
• Structural basis for PTPA interaction with the invariant C-terminal tail of PP2A.
  Löw C, Quistgaard EM, Kovermann M, Anandapadamanaban M, Balbach J, Nordlund P.
  Biol. Chem. 2014, 395:881-889.

Publications in 2013

• Molecular function of the prolyl cis/trans isomerase and metallochaperone SlyD.
  Kovermann M, Schmid FX, Balbach J.
  Biol. Chem. 2013, 394:965-975.
   
• Dynamic control of the prolyl isomerase function of the dual-domain SlyD protein.
  Kovermann M, Balbach J.
  Biophys. Chem. 2013, 171:16-23.

Publications in 2012

• Optimisation of over-expression in E. coli and biophysical characterisation of human membrane protein synaptogyrin 1.
  Löw C, Jegerschöld C, Kovermann M, Moberg P, Nordlund P.
  PLoS One 2012,7:e38244.
   
• Real-time observation of polymer network formation by liquid- and solid-state NMR revealing multistage reaction kinetics.
  Kovermann M, Saalwächter K, Chassé W. J.
  Phys. Chem. B 2012, 116:7566-7574.
   
• New binding mode to TNF-alpha revealed by ubiquitin-based artificial binding protein.
  Hoffmann A, Kovermann M, Lilie H, Fiedler M, Balbach J, Rudolph R, Pfeifer S.
  PLoS One 2012, 7:e31298.

Publications in 2011

• Escherichia coli peptide binding protein OppA has a preference for positively charged peptides.
  Klepsch MM, Kovermann M, Löw C, Balbach J, Permentier HP, Fusetti F, de Gier JW, Slotboom DJ, Berntsson RP.
  J. Mol. Biol. 2011, 414:75-85.
   
• Local and coupled thermodynamic stability of the two-domain and bifunctional enzyme SlyD from Escherichia coli.
  Haupt C, Weininger U, Kovermann M, Balbach J.
  Biochemistry 2011, 50:7321-7329.
   
• Transient enzyme-substrate recognition monitored by real-time NMR.
  Haupt C, Patzschke R, Weininger U, Gröger S, Kovermann M, Balbach J.
  J. Am. Chem. Soc. 2011, 133:11154-11162.
   
• Conformational plasticity and dynamics in the generic protein folding catalyst SlyD unraveled by single-molecule FRET.
  Kahra D, Kovermann M, Löw C, Hirschfeld V, Haupt C, Balbach J, Hübner CG.
  J. Mol. Biol. 2011, 411:781-790.
   
• NMR relaxation unravels interdomain crosstalk of the two domain prolyl isomerase and chaperone SlyD.
  Kovermann M, Zierold R, Haupt C, Löw C, Balbach J.
  Biochim. Biophys. Acta. 2011, 1814:873-881.
   
• The presence of membranes or micelles induces structural changes of the myristoylated guanylate-cyclase activating protein-2.
  Theisgen S, Thomas L, Schröder T, Lange C, Kovermann M, Balbach J, Huster D.
  Eur. Biophys. J. 2011, 40:565-576.

Publications in 2010

• DHPC strongly affects the structure and oligomerization propensity of Alzheimer's Aβ(1-40) peptide.
  Dahse K, Garvey M, Kovermann M, Vogel A, Balbach J, Fändrich M, Fahr A.
  J. Mol. Biol. 2010, 403:643-659.
   
• The prolyl isomerase domain of PpiD from Escherichia coli shows a parvulin fold but is devoid of catalytic activity.
  Weininger U, Jakob RP, Kovermann M, Balbach J, Schmid FX.
  Protein Sci. 2010, 19:6-18.

Publications in 2009

• Coordination chemistry approach to the long-standing challenge of stereocontrolled chemical glycosylation.
  Pornsuriyasak P, Vetter C, Kaeothip S, Kovermann M, Balbach J, Steinborn D, Demchenko AV.
  Chem. Commun. 2009, 42:6379-6381.
   
• NMR solution structure of SlyD from Escherichia coli: spatial separation of prolyl isomerase and chaperone function.
  Weininger U, Haupt C, Schweimer K, Graubner W, Kovermann M, Brüser T, Scholz C, Schaarschmidt P, Zoldak G, Schmid FX, Balbach J.
  J. Mol. Biol. 2009, 387:295-305.

Publications in 2008

• Slow dynamics in glassy methyl alpha-l-rhamnopyranoside studied by 1D NMR exchange experiments.
  Reichert D, Kovermann M, Hunter N, Hughes D, Pascui O, Belton P.
  Phys. Chem. Chem. Phys. 2008, 10:542-549.